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Protocols Involving Cells dendritic cell protocols obtaining dendritic cells from mice obtaining thyioglycalated Macrophages from mice obtaining spleenocytes from mice MOLT-4 & THP1 cells MT-2 cells When suspensions of mouse bone marrow are cultured in the presence of GM-CSF, 3 types of myeloid cells expand in numbers (a) neutrophils predominate but do not adhere to the culture surface. (b) Macrophages are firmly adherent to the culture vessel. (c) dendritic cells arise from cellular aggregates that are attached to the marrow stroma. The aggregates become covered with sheet-like cell processes and eventually release typical single dendritic cells. |
