(Apple)-cellquest Pro

File-open (look for Ray)

Ray-Jim (look for template)

opendoc-

Acquire-connect to Cytometer (brings up page)

Change-put your name + new forlder (date) to create new folder. choose that folder

Acquire-Acquisitions & Storage (10000)

cytometer-threshold (FSC-H, None)

Cytometer=instrument settings

Open-look for settings

Set (will see wheel come up) make sure setings unchecked (thersie takes all cells)

Operation

take water bue out/put in your sample

--sample ID-Label (ex. Day -_

Acquire (under opended acquisition control)

take next sample (tap on bottom)

sample ID

Acquire

keep reating

Closing

fun bleach then 5' minutes

then water

shut computer off, then machine

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